| 质粒名称: | AAVS1-Puro CAG FUCCI |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 136934 |
| 存储实验室: | Xiaoping Bao |
|
载体骨架基本信息 |
|
| 载体名称: | AAVS1 Puro CAG |
| 载体出品公司: | Addgene Plasmid #80945 |
| 空载体大小: | 10235 bp |
| 完整质粒大小: | 12890 bp |
| 载体类型: | 哺乳动物表达，合成生物学 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | Ampicillin, 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)；FUCCI |
| 物种来源: | sapiens (human), Synthetic |
| 插入大小: | 2655 bp |
| 启动子: | CAG |
| 标签/融合蛋白: |
Clover (N terminal on insert)
mKO2 (N terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SalI (not destroyed) |
| 3’克隆位点: | MluI (not destroyed) |
| 5’测序引物 | gctaaccatgttcatgccttc |
| 3’测序引物 | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | AAVS1-Puro CAG FUCCI was a gift from Xiaoping Bao (Addgene plasmid # 136934 ; http://n2t.net/addgene:136934 ; RRID:Addgene_136934) |
| 参考文献部分: | Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells. Chang Y, Hellwarth PB, Randolph LN, Sun Y, Xing Y, Zhu W, Lian XL, Bao X. Biotechnol Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352. 10.1002/bit.27352 PubMed 32277708 |
买家导航
AAVS1-Puro CAG FUCCI载体质粒基本信息
AAVS1-Puro CAG FUCCI质粒图谱载体图谱和AAVS1-Puro CAG FUCCI载体序列质粒序列多克隆位点信息
AAVS1-Puro CAG FUCCI质粒载体简介
AAVS1-Puro CAG FUCCI质粒序列载体序列
LOCUS AAVS1-Puro CAG FUCCI 12849 bp DNA circular SYN 15-DEC-2025
DEFINITION donor plasmid for constitutive FUCCI expression in human cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12849)
AUTHORS Chang Y, Hellwarth PB, Randolph LN, Sun Y, Xing Y, Zhu W, Lian XL,
Bao X
TITLE Fluorescent indicators for continuous and lineage-specific reporting
of cell-cycle phases in human pluripotent stem cells.
JOURNAL Biotechnol Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352.
PUBMED 32277708
REFERENCE 2 (bases 1 to 12849)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352."
COMMENT Sequence Label: AAVS1-Puro CAG FUCCI
FEATURES Location/Qualifiers
source 1..12849
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 31..834
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 841..866
/label=SA
/note="splice acceptor site"
CDS 890..943
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 953..1552
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(953..972)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1449..1469
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind complement(1584..1601)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1590..1814
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
enhancer 1820..2199
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 2435..3451
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 3375..3397
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3459..3478
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS join(3521..3523,3524..3526,3527..4237)
/codon_start=1
/product="bright green-yellow fluorescent protein derived
from GFP (Lam et al., 2012)"
/label=Clover
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(3566..3587)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind 4174..4195
/label=EGFP-C
/note="EGFP, forward primer"
CDS 4268..4597
/codon_start=1
/product="degron consisting of residues 1-110 of human
Geminin (Zielke and Edgar, 2015)"
/label=Gem1 (1-110)
/translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
KRRKAL"
misc_feature 4602..5174
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4769..4786)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4996..5015
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5189..5839
/codon_start=1
/product="monomeric Kusabira-Orange 2 fluorescent protein"
/label=mKO2
/translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
CDS 5189..5839
/codon_start=1
/product="humanized monomeric Kusabira-Orange fluorescent
protein"
/label=hmKO
/note="mammalian codon-optimized"
/translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
CDS 5894..6166
/codon_start=1
/product="degron consisting of residues 30-120 of human
Cdt1 (Zielke and Edgar, 2015)"
/label=Cdt1 (30-120)
/translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
misc_feature 6204..6792
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6257..6277)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6675..6686)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 6824..7300
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(7023..7042)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
primer_bind complement(7410..7429)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 7475..7530
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(7529..7548)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(7891..7907)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 7915..7931
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(7939..7945,7946..7963,7964..7969))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7984..8005
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 8079..8915
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(8949..8968)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8950..8968)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8975..8992)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8975..8991)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8984..9006)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS 9129..9431
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 9310..9329
/label=ccdB-fwd
/note="ccdB gene, forward primer"
primer_bind complement(9834..9853)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 10448..10467
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
CDS complement(join(10828..11619,11620..11688))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11451..11470
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11812..12400
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 12301..12320
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12554..12571
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 12688..12709
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(12724..12729,12730..12747,12748..12754)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12762..12778
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 12786..12802
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 12823..12841
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 agtcctgcag gtttaaacga attcgccctt tgctttctct gaccagcatt ctctcccctg
61 ggcctgtgcc gctttctgtc tgcagcttgt ggcctgggtc acctctacgg ctggcccaga
121 tccttccctg ccgcctcctt caggttccgt cttcctccac tccctcttcc ccttgctctc
181 tgctgtgttg ctgcccaagg atgctctttc cggagcactt ccttctcggc gctgcaccac
241 gtgatgtcct ctgagcggat cctccccgtg tctgggtcct ctccgggcat ctctcctccc
301 tcacccaacc ccatgccgtc ttcactcgct gggttccctt ttccttctcc ttctggggcc
361 tgtgccatct ctcgtttctt aggatggcct tctccgacgg atgtctccct tgcgtcccgc
421 ctccccttct tgtaggcctg catcatcacc gtttttctgg acaaccccaa agtaccccgt
481 ctccctggct ttagccacct ctccatcctc ttgctttctt tgcctggaca ccccgttctc
541 ctgtggattc gggtcacctc tcactccttt catttgggca gctcccctac cccccttacc
601 tctctagtct gtgctagctc ttccagcccc ctgtcatggc atcttccagg ggtccgagag
661 ctcagctagt cttcttcctc caacccgggc ccctatgtcc acttcaggac agcatgtttg
721 ctgcctccag ggatcctgtg tccccgagct gggaccacct tatattccca gggccggtta
781 atgtggctct ggttctgggt acttttatct gtcccctcca ccccacagtg gggcaagctt
841 ctgacctctt ctcttcctcc cacagggcct cgagagatct ggcagcggag agggcagagg
901 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctaggctcg agatgaccga
961 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
1021 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
1081 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
1141 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
1201 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
1261 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
1321 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
1381 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
1441 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
1501 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatctagagg
1561 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
1621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
1681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
1741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
1801 ggtgggctct atgggtctcg acattgatta ttgactagtt attaatagta atcaattacg
1861 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
1921 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
1981 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
2041 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
2101 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
2161 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
2221 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
2281 taattatttt gtgcagcgat gggggcgggg gggggggggg gcgaggcgga gaggtgcggc
2341 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
2401 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
2461 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
2521 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
2581 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
2641 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
2701 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
2761 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
2821 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
2881 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
2941 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
3001 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
3061 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
3121 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
3181 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
3241 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
3301 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
3361 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
3421 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
3481 gtctcatcat tttggcaaag aattcgtcga cgctagcacc atggtgagca agggcgagga
3541 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
3601 gttcagcgtc cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
3661 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
3721 cggcgtggcc tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
3781 cgccatgccc gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta
3841 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
3901 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa
3961 cagccacaac gtctatatca cggccgacaa gcagaagaac ggcatcaagg ctaacttcaa
4021 gatccgccac aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
4081 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccgc
4141 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
4201 cgccgggatt acacatggca tggacgagct gtacaaggat atccatgcac actggcggcc
4261 gctcgagatg aatcccagta tgaagcagaa acaagaagaa atcaaagaga atataaagaa
4321 tagttctgtc ccaagaagaa ctctgaagat gattcagcct tctgcatctg gatctcttgt
4381 tggaagagaa aatgagctgt ccgcaggctt gtccaaaagg aaacatcgga atgaccactt
4441 aacatctaca acttccagcc ctggggttat tgtcccagaa tctagtgaaa ataaaaatct
4501 tggaggagtc acccaggagt catttgatct tatgattaaa gaaaatccat cctctcagta
4561 ttggaaggaa gtggcagaaa aacggagaaa ggcgctgtaa gcccctctcc ctcccccccc
4621 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
4681 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
4741 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
4801 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
4861 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
4921 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
4981 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
5041 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
5101 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
5161 aaacacgatg ataatggcca cagaattcat ggtgagcgtg atcaagcccg agatgaagat
5221 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaatcgagg gtgagggcac
5281 aggcagacct tacgagggac atcaggagat gacactgcgc gtcacaatgg ccgagggcgg
5341 gccaatgcct ttcgccttcg acctggtgtc ccacgtgttc tgttacggcc acagagtttt
5401 taccaagtac ccagaagaga tcccagacta tttcaagcag gcctttcctg agggcctgtc
5461 ctgggagagg tccctggagt tcgaggacgg cggctccgcc tccgtgagcg cccacatcag
5521 cctgaggggc aacaccttct accacaagtc caagttcacc ggcgtgaact tccccgccga
5581 cggccccatc atgcagaacc agagcgtgga ctgggagccc tccaccgaga agatcaccgc
5641 cagcgacggc gtgctgaagg gcgacgtgac catgtacctg aagctggagg gcggcggcaa
5701 ccacaagtgc cagatgaaga ccacctacaa ggccgccaag gagatcctgg agatgcccgg
5761 cgaccactac atcggccaca ggctggtgag gaagaccgag ggcaacatca ccgagcaggt
5821 ggaggacgcc gtggcccact actcaatgtt gccttccctg ggcgatatcc atgcacactg
5881 gcggccgctc gagcccagcc ccgccaggcc cgcactccgc gccccggcct ccgctaccag
5941 tggcagccgc aagcgcgccc gcccgcccgc cgcccccgga cgcgaccagg ccaggccacc
6001 ggcccgcagg agactgcggc tgtcggtgga cgaggtttcc agccccagta cccccgaggc
6061 cccagacatc ccagcctgcc cttctccggg ccagaagata aagaaatcca ccccggcagc
6121 aggtcagccg ccccacctga catccgcgca ggaccaggac accatctgag tttacgcgtg
6181 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
6241 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
6301 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
6361 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
6421 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
6481 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
6541 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
6601 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
6661 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
6721 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
6781 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga
6841 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
6901 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
6961 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
7021 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
7081 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
7141 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
7201 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
7261 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccag
7321 gcgcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
7381 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
7441 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
7501 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
7561 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
7621 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
7681 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
7741 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
7801 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
7861 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
7921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
7981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
8041 aaacctgtcg tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca
8101 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
8161 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
8221 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
8281 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
8341 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
8401 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
8461 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
8521 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
8581 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
8641 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
8701 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
8761 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
8821 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
8881 tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat
8941 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
9001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
9061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
9121 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
9181 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
9241 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
9301 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
9361 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
9421 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
9481 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
9541 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
9601 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
9661 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
9721 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
9781 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
9841 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
9901 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
9961 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
10021 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
10081 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
10141 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
10201 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
10261 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
10321 gcgaggatct cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa
10381 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
10441 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
10501 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
10561 cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt
10621 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
10681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc
10741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
10801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
10861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
10921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
10981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
11041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
11101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
11161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
11221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
11281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
11341 tgtcatgcca tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg
11401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
11461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
11521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
11581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
11641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
11701 tcaatattat tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga
11761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
11821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
11881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
11941 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
12001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
12061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
12121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
12181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
12241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
12301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
12361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
12421 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
12481 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
12541 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
12601 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
12661 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
12721 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
12781 tcacacagga aacagctatg accatgatta cgccaagctc agaattaacc ctcactaaag
12841 ggactagct
//
AAVS1-Puro CAG FUCCI载体图谱质粒图谱pdf版和AAVS1-Puro CAG FUCCI载体序列质粒序列等相关资料下载
AAVS1-Puro CAG FUCCI质粒载体应用举例
- 上一篇：pMK395 (AAVS1 CMV-OsTIR1(F74A))
- 下一篇：没有了
